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Institutional Repository [SANDBOX]
Technical University of Crete
Technical University of Crete
EN
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| URI: | http://purl.tuc.gr/dl/dias/68DFA558-1486-4941-89CF-C28A6B6C7110 | ||
| Year | 2023 | ||
| Type of Item | Diploma Work | ||
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| Bibliographic Citation | Konstantina Pantelidaki, "Selection of the appropriate strain of microalgae Stichococcus sp. with characteristics of reduced chlorophyll and higher lipid production", Diploma Work, School of Chemical and Environmental Engineering, Technical University of Crete, Chania, Greece, 2023 https://doi.org/10.26233/heallink.tuc.94912 | ||
| Appears in Collections |
The aim of the present study is to maximize microalgae growth, by reducing the "shadow effect" created by the high concentration of chlorophyll in the cell mass. To achieve the above, a mutagenic agent was applied to Stichococcus sp. and strains with low chlorophyll content were selected. These strains were then tested for growth productivity and their ability to produce lipids and pigments. Stichococcus, is a green rod-shaped microalgae, which belongs to the classification of chlorophytes. It is resistant to extreme pH values, high temperatures, salinity and nutrients in its growing environment. It grows mainly in saline water, but researchers have encountered it in fresh water, soil, and even in the extreme Antarctic environment. The mutation of Stichococcus sp. was obtained using Ethyl methanesulfonate (EMS) as a chemical mutagen. EMS is an ethylating agent, which causes changes in the DNA of microalgae. Results showed that the chlorophyll content was successfully reduced in the mutant strains, named EMS1 and EMS2.
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