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Ιδρυματικό Αποθετήριο [SANDBOX]
Πολυτεχνείο Κρήτης
Πολυτεχνείο Κρήτης
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| URI: | http://purl.tuc.gr/dl/dias/89FC39C3-64A9-4BF5-9EEE-9A18E29468F4 | ||
| Έτος | 2016 | ||
| Τύπος | Δημοσίευση σε Περιοδικό με Κριτές | ||
| Άδεια Χρήσης |
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| Βιβλιογραφική Αναφορά | K. C. Tsolis, E. Bagli, K. Kanaki, S. Zografou, S. Carpentier, E. S. Bei, S. Christoforidis, M. Zervakis, C. Murphy, T. Fotsis and A. Economou, "Proteome changes during transition from human embryonic to vascular progenitor cells," J. Proteome Res., vol. 15, no. 6, pp. 1995-2007, Jun. 2016. doi: 10.1021/acs.jproteome.6b00180 https://doi.org/10.1021/acs.jproteome.6b00180 | ||
| Εμφανίζεται στις Συλλογές |
Human embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to generate tight vessels by differentiating hESCs in CD34+ vascular progenitor cells using chemically defined media and growth conditions. We selectively purified these cells from CD34- outgrowth populations also formed. To analyze these differentiation processes, we compared the proteomes of the hESCs with those of the CD34+ and CD34- populations using high resolution mass spectrometry, label-free quantification, and multivariate analysis. Eighteen protein markers validate the differentiated phenotypes in immunological assays; nine of these were also detected by proteomics and show statistically significant differential abundance. Another 225 proteins show differential abundance between the three cell types. Sixty-three of these have known functions in CD34+ and CD34- cells. CD34+ cells synthesize proteins implicated in endothelial cell differentiation and smooth muscle formation, which support the bipotent phenotype of these progenitor cells. CD34- cells are more heterogeneous synthesizing muscular/osteogenic/chondrogenic/adipogenic lineage markers. The remaining >150 differentially abundant proteins in CD34+ or CD34- cells raise testable hypotheses for future studies to probe vascular morphogenesis.
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