Institutional Repository [SANDBOX]
Technical University of Crete
EN  |  EL

Search

Browse

My Space

Substrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cells

Mylonaki Eleni, Dailiana, Z., Trepat, X., Lagoudakis Michael

Simple record


URIhttp://purl.tuc.gr/dl/dias/A807AD7B-4F26-4A45-B27C-46C8B54C5CFF-
Identifierhttp://thales.iacm.forth.gr/~emilona/MylonaESBMEProceedings08.pdf-
Languageen-
Extent4 pagesen
TitleSubstrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cellsen
CreatorMylonaki Elenien
CreatorΜυλωνακη Ελενηel
CreatorDailiana, Z.en
CreatorTrepat, X.en
CreatorLagoudakis Michaelen
CreatorΛαγουδακης Μιχαηλel
Content SummaryChemical cues and physical forces tightly control the milieu where a cell is born, survives, and finally dies. Of the physical forces, tissue rigidity is known to direct cell fate. Nonetheless, malignant tumors drastically alter the local physiological tissue rigidity. Our aim was to determine the functional changes of cells derived from an osteosarcoma, a very stiff malignant formation, to substrates of varying rigidity. Cells extracted from a human femoral osteosarcoma were exposed to collagen I-coated polyacrylamide substrates mimicking the rigidity of brain (1 kPa), connective tissue (7 kPa), and collagenous bone (55 kPa). Glass was used as a control substrate. Osteosarcoma cells occupied the smallest area and were rounder when on the compliant 1 kPa substrate compared to the stiffer substrates. Additionally, cell apoptosis, as assessed by Annexin V staining, and total death rate were significantly increased on the more compliant substrates of 1 and 7 kPa. Finally, as computed via a Fourier transform algorithm, cells exerted greater traction forces on the rigid 55 kPa substratum and less on the more compliant 7 and 1 kPa substrates. Taken together, these data suggest that osteosarcoma cells survive and function more efficiently on substrates mechanically closer to their native microenvironment. en
Type of ItemΠλήρης Δημοσίευση σε Συνέδριοel
Type of ItemConference Full Paperen
Licensehttp://creativecommons.org/licenses/by/4.0/en
Date of Item2015-11-13-
Date of Publication2008-
SubjectBio-process engineeringen
SubjectBioprocess engineeringen
Subjectbiochemical engineeringen
Subjectbio process engineeringen
Subjectbioprocess engineeringen
Bibliographic CitationEl. Mylona, Z. Dailiana, X. Trepat, and M. G. Lagoudakis. (2008, June). Substrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cells. [Online]. Available: http://thales.iacm.forth.gr/~emilona/MylonaESBMEProceedings08.pdfen

Services

Statistics