URI | http://purl.tuc.gr/dl/dias/A807AD7B-4F26-4A45-B27C-46C8B54C5CFF | - |
Identifier | http://thales.iacm.forth.gr/~emilona/MylonaESBMEProceedings08.pdf | - |
Language | en | - |
Extent | 4 pages | en |
Title | Substrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cells | en |
Creator | Mylonaki Eleni | en |
Creator | Μυλωνακη Ελενη | el |
Creator | Dailiana, Z. | en |
Creator | Trepat, X. | en |
Creator | Lagoudakis Michael | en |
Creator | Λαγουδακης Μιχαηλ | el |
Content Summary | Chemical cues and physical forces tightly
control the milieu where a cell is born, survives, and
finally dies. Of the physical forces, tissue rigidity is
known to direct cell fate. Nonetheless, malignant
tumors drastically alter the local physiological tissue
rigidity. Our aim was to determine the functional
changes of cells derived from an osteosarcoma, a
very stiff malignant formation, to substrates of varying
rigidity. Cells extracted from a human femoral
osteosarcoma were exposed to collagen I-coated
polyacrylamide substrates mimicking the rigidity of
brain (1 kPa), connective tissue (7 kPa), and collagenous
bone (55 kPa). Glass was used as a control
substrate. Osteosarcoma cells occupied the smallest
area and were rounder when on the compliant 1 kPa
substrate compared to the stiffer substrates. Additionally,
cell apoptosis, as assessed by Annexin V
staining, and total death rate were significantly increased
on the more compliant substrates of 1 and 7
kPa. Finally, as computed via a Fourier transform
algorithm, cells exerted greater traction forces on the
rigid 55 kPa substratum and less on the more compliant
7 and 1 kPa substrates. Taken together, these
data suggest that osteosarcoma cells survive and
function more efficiently on substrates mechanically
closer to their native microenvironment. | en |
Type of Item | Πλήρης Δημοσίευση σε Συνέδριο | el |
Type of Item | Conference Full Paper | en |
License | http://creativecommons.org/licenses/by/4.0/ | en |
Date of Item | 2015-11-13 | - |
Date of Publication | 2008 | - |
Subject | Bio-process engineering | en |
Subject | Bioprocess engineering | en |
Subject | biochemical engineering | en |
Subject | bio process engineering | en |
Subject | bioprocess engineering | en |
Bibliographic Citation | El. Mylona, Z. Dailiana, X. Trepat, and M. G. Lagoudakis. (2008, June). Substrate rigidity dictates phenotype, survival, and mechanics of primary human osteosarcoma cells. [Online]. Available: http://thales.iacm.forth.gr/~emilona/MylonaESBMEProceedings08.pdf | en |