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| URI | http://purl.tuc.gr/dl/dias/538DD8FB-D775-48A7-910B-859FD996D229 | - |
| Γλώσσα | en | - |
| Μέγεθος | 11 | en |
| Τίτλος | Bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites | en |
| Δημιουργός | Katsivela E. | en |
| Δημιουργός | Moore E. R. B. | en |
| Δημιουργός | Maroukli D. | en |
| Δημιουργός | Strömpl C. | en |
| Δημιουργός | Pieper D. | en |
| Δημιουργός | Kalogerakis Nikos | en |
| Δημιουργός | Καλογερακης Νικος | el |
| Περιγραφή | Δημοσίευση σε επιστημονικό περιοδικό | el |
| Περίληψη | In-situ bioremediation of petroleum waste sludge in landfarming sites of Motor Oil Hellas (petroleum refinery) was studied by monitoring the changes of the petroleum composition of the waste sludge, as well as the changes in the structure of the microbial community, for a time period of 14 months. The analyses indicated an enhanced degradation of the petroleum hydrocarbons in the landfarming areas. A depletion of n-alkanes of approximately 75–100% was obtained. Marked changes of the microbial communities of the landfarms occurred concomitantly with the degradation of the petroleum hydrocarbons. The results obtained from terminal restriction fragment length polymorphism (T-RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rRNA genes demonstrated that bacteria originating from the refinery waste sludge and newly selected bacteria dominated the soil bacterial community during the period of the highest degradation activity. However, the diversity of the microbial community was decreased with increased degradation of the petroleum hydrocarbons contained in the landfarms. T-RFLP fingerprints of bacteria of the genera Enterobacter and Ochrobactrum were detected in the landfarmed soil over the entire treatment period of 14 months. In contrast, the genus Alcaligenes appeared in significant numbers only within the 10 month old landfarmed soil. Genes encoding catechol 2,3-dioxygenase (subfamily I.2.A) were detected only in DNA of the untreated refinery waste sludge. However, none of the genes known to encode the enzymes alkane hydroxylase AlkB, catechol 2,3-dioxygenase (subfamily I.2.A) and naphthalene dioxygenase nahAc could be detected in DNA of the landfarmed soils. | en |
| Τύπος | Peer-Reviewed Journal Publication | en |
| Τύπος | Δημοσίευση σε Περιοδικό με Κριτές | el |
| Άδεια Χρήσης | http://creativecommons.org/licenses/by/4.0/ | en |
| Ημερομηνία | 2015-10-18 | - |
| Ημερομηνία Δημοσίευσης | 2005 | - |
| Θεματική Κατηγορία | bacterial community dynamics | en |
| Θεματική Κατηγορία | in-situ biodegradation | en |
| Θεματική Κατηγορία | petroleum hydrocarbons | en |
| Θεματική Κατηγορία | refinery waste sludge | en |
| Θεματική Κατηγορία | 16S rDNA | en |
| Θεματική Κατηγορία | T-RFLP fingerprinting | en |
| Βιβλιογραφική Αναφορά | E. Katsivela, E.R.B. Moore, D. Maroukli, C. Strömpl, D. Pieper and N. Kalogerakis, "Bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites," Biodegradation, vol. 16, no. 2, pp. 169-180, Mar. 2005. | en |
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