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Bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites

Katsivela E. , Moore E. R. B., Maroukli D., Strömpl C. , Pieper D. , Kalogerakis Nikos

Απλή Εγγραφή


URIhttp://purl.tuc.gr/dl/dias/538DD8FB-D775-48A7-910B-859FD996D229-
Γλώσσαen-
Μέγεθος11en
ΤίτλοςBacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sitesen
ΔημιουργόςKatsivela E. en
Δημιουργός Moore E. R. B.en
Δημιουργός Maroukli D.en
ΔημιουργόςStrömpl C. en
ΔημιουργόςPieper D. en
ΔημιουργόςKalogerakis Nikosen
ΔημιουργόςΚαλογερακης Νικοςel
ΠεριγραφήΔημοσίευση σε επιστημονικό περιοδικό el
ΠερίληψηIn-situ bioremediation of petroleum waste sludge in landfarming sites of Motor Oil Hellas (petroleum refinery) was studied by monitoring the changes of the petroleum composition of the waste sludge, as well as the changes in the structure of the microbial community, for a time period of 14 months. The analyses indicated an enhanced degradation of the petroleum hydrocarbons in the landfarming areas. A depletion of n-alkanes of approximately 75–100% was obtained. Marked changes of the microbial communities of the landfarms occurred concomitantly with the degradation of the petroleum hydrocarbons. The results obtained from terminal restriction fragment length polymorphism (T-RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rRNA genes demonstrated that bacteria originating from the refinery waste sludge and newly selected bacteria dominated the soil bacterial community during the period of the highest degradation activity. However, the diversity of the microbial community was decreased with increased degradation of the petroleum hydrocarbons contained in the landfarms. T-RFLP fingerprints of bacteria of the genera Enterobacter and Ochrobactrum were detected in the landfarmed soil over the entire treatment period of 14 months. In contrast, the genus Alcaligenes appeared in significant numbers only within the 10 month old landfarmed soil. Genes encoding catechol 2,3-dioxygenase (subfamily I.2.A) were detected only in DNA of the untreated refinery waste sludge. However, none of the genes known to encode the enzymes alkane hydroxylase AlkB, catechol 2,3-dioxygenase (subfamily I.2.A) and naphthalene dioxygenase nahAc could be detected in DNA of the landfarmed soils.en
ΤύποςPeer-Reviewed Journal Publicationen
ΤύποςΔημοσίευση σε Περιοδικό με Κριτέςel
Άδεια Χρήσηςhttp://creativecommons.org/licenses/by/4.0/en
Ημερομηνία2015-10-18-
Ημερομηνία Δημοσίευσης2005-
Θεματική Κατηγορίαbacterial community dynamicsen
Θεματική Κατηγορίαin-situ biodegradationen
Θεματική Κατηγορίαpetroleum hydrocarbonsen
Θεματική Κατηγορίαrefinery waste sludge en
Θεματική Κατηγορία16S rDNA en
Θεματική ΚατηγορίαT-RFLP fingerprintingen
Βιβλιογραφική ΑναφοράE. Katsivela, E.R.B. Moore, D. Maroukli, C. Strömpl, D. Pieper and N. Kalogerakis, "Bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites," Biodegradation, vol. 16, no. 2, pp. 169-180, Mar. 2005. en

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