URI | http://purl.tuc.gr/dl/dias/4B0C01C5-535D-459F-8C2C-21EDA89873B1 | - |
Αναγνωριστικό | https://doi.org/10.1016/j.scitotenv.2015.02.036 | - |
Γλώσσα | en | - |
Μέγεθος | 10 pages | en |
Τίτλος | Sorption of Pseudomonas putida onto differently structured kaolinite minerals | en |
Δημιουργός | Chrysikopoulos Constantinos | en |
Δημιουργός | Χρυσικοπουλος Κωνσταντινος | el |
Δημιουργός | Maria I. Bellou | en |
Δημιουργός | Maria A. Tselepi | en |
Δημιουργός | Vasiliki I. Syngouna | en |
Δημιουργός | Petros A. Kokkinos | en |
Δημιουργός | Apostolos Vantarakis | en |
Δημιουργός | Spyros C. Paparrodopoulos | en |
Περίληψη | Human adenoviruses (hAdVs) are pathogenic viruses responsible for public health problems worldwide. They
have also been used as viral indicators in environmental systems. Coliphages (e.g., MS2, ΦX174) have also
been studied as indicators of viral pollution in fecally contaminated water. Our objective was to evaluate the
distribution of three viral fecal indicators (hAdVs, MS2, and ΦΧ174), between two different phyllosilicate clays
(kaolinite and bentonite) and the aqueous phase. A series of static and dynamic experiments were conducted
under two different temperatures (4, 25 °C) for a time period of seven days. HAdV adsorption was examined
in DNase I reaction buffer (pH=7.6, and ionic strength (IS)=1.4 mM), whereas coliphage adsorption in phosphate
buffered saline solution (pH=7, IS=2 mM). Moreover, the effect of IS on hAdV adsorption under static
conditions was evaluated. The adsorption of hAdVwas assessed by real-time PCR and its infectivitywas tested by
cultivation methods. The coliphages MS2 and ΦΧ174 were assayed by the double-layer overlay method. The
experimental results have shown that coliphage adsorption onto both kaolinite and bentonite was higher for
the dynamic than the static experiments; whereas hAdV adsorption was lower under dynamic conditions. The
adsorption of hAdV increased with decreasing temperature, contrary to the results obtained for the coliphages.
This study examines the combined effect of temperature, agitation, clay type, and IS on hAdV adsorption onto
clays. The results provide useful new information on the effective removal of viral fecal indicators (MS2,
ΦX174 and hAdV) from dilute aqueous solutions by adsorption onto kaolinite and bentonite | en |
Τύπος | Peer-Reviewed Journal Publication | en |
Τύπος | Δημοσίευση σε Περιοδικό με Κριτές | el |
Άδεια Χρήσης | http://creativecommons.org/licenses/by/4.0/ | en |
Ημερομηνία | 2015-09-23 | - |
Ημερομηνία Δημοσίευσης | 2015 | - |
Βιβλιογραφική Αναφορά | M. I. Bellou ,V. I. Syngouna , M. A. Tselepi , P. A. Kokkinos , S. C. Paparrodopoulos ,A. Vantarakis , C. V. Chrysikopoulos , "Interaction of human adenoviruses and coliphages with kaolinite
and bentonite " ,Scien. of the Tot. Envir.,vol. 517 ,pp.86–95,2015.doi:10.1016/j.scitotenv.2015.02.036 | en |