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Institutional Repository [SANDBOX]
Technical University of Crete
Technical University of Crete
EN
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| URI: | http://purl.tuc.gr/dl/dias/4B0C01C5-535D-459F-8C2C-21EDA89873B1 | ||
| Year | 2015 | ||
| Type of Item | Peer-Reviewed Journal Publication | ||
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| Bibliographic Citation | M. I. Bellou ,V. I. Syngouna , M. A. Tselepi , P. A. Kokkinos , S. C. Paparrodopoulos ,A. Vantarakis , C. V. Chrysikopoulos , "Interaction of human adenoviruses and coliphages with kaolinite and bentonite " ,Scien. of the Tot. Envir.,vol. 517 ,pp.86–95,2015.doi:10.1016/j.scitotenv.2015.02.036 https://doi.org/10.1016/j.scitotenv.2015.02.036 | ||
| Appears in Collections |
Human adenoviruses (hAdVs) are pathogenic viruses responsible for public health problems worldwide. Theyhave also been used as viral indicators in environmental systems. Coliphages (e.g., MS2, ΦX174) have alsobeen studied as indicators of viral pollution in fecally contaminated water. Our objective was to evaluate thedistribution of three viral fecal indicators (hAdVs, MS2, and ΦΧ174), between two different phyllosilicate clays(kaolinite and bentonite) and the aqueous phase. A series of static and dynamic experiments were conductedunder two different temperatures (4, 25 °C) for a time period of seven days. HAdV adsorption was examinedin DNase I reaction buffer (pH=7.6, and ionic strength (IS)=1.4 mM), whereas coliphage adsorption in phosphatebuffered saline solution (pH=7, IS=2 mM). Moreover, the effect of IS on hAdV adsorption under staticconditions was evaluated. The adsorption of hAdVwas assessed by real-time PCR and its infectivitywas tested bycultivation methods. The coliphages MS2 and ΦΧ174 were assayed by the double-layer overlay method. Theexperimental results have shown that coliphage adsorption onto both kaolinite and bentonite was higher forthe dynamic than the static experiments; whereas hAdV adsorption was lower under dynamic conditions. Theadsorption of hAdV increased with decreasing temperature, contrary to the results obtained for the coliphages.This study examines the combined effect of temperature, agitation, clay type, and IS on hAdV adsorption ontoclays. The results provide useful new information on the effective removal of viral fecal indicators (MS2,ΦX174 and hAdV) from dilute aqueous solutions by adsorption onto kaolinite and bentonite
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