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Institutional Repository [SANDBOX]
Technical University of Crete
Technical University of Crete
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| URI: | http://purl.tuc.gr/dl/dias/964270B5-4ECC-4F30-A47F-77EB8E8FA359 | ||
| Year | 2013 | ||
| Type of Item | Peer-Reviewed Journal Publication | ||
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| Bibliographic Citation | C. V. Chrysikopoulos, I.D. Manariotis, V. I. Syngouna, "Virus inactivation by high frequency ultrasound in combination with visible light ",Col. and Surf. B: Biointerf., vol. 107 ,pp. 174– 179,2013.doi: 10.1016/j.colsurfb.2013.01.038 https://doi.org/10.1016/j.colsurfb.2013.01.038 | ||
| Appears in Collections |
In this study, the effects of high frequency ultrasound (US) and visible light (VL) on virus inactivationwere investigated. The bacteriophages X174 and MS2 were used as model viruses. The experimentswere performed at room temperature at three different, relatively high US frequencies (i.e., 582, 862, and1142 kHz) with and without the use of VL, and different initial virus concentrations. The two bacteriophageswere diluted in phosphate-buffered saline solution to a titer of 103–104 pfu/mL. The experimentalvirus inactivation data were satisfactorily represented by a simple first-order kinetic expression. Virusinactivation was faster at the lower frequencies (582 and 862 kHz). Furthermore, it was observed thatMS2 was inactivated faster than X174. The simultaneous use of US and VL was found to be more effectivethan US alone for MS2 inactivation, indicating the existence of a synergistic effect
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