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Transport of pseudomonas putida in a 3-D bench scale experimental aquifer

Chrysikopoulos Constantinos, Vasileios E. Katzourakis, Ioanna A. Vasiliadou, Vasiliki I. Syngouna

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URI: http://purl.tuc.gr/dl/dias/5BAF6094-BD73-4BD3-AB12-B9B9E0A3F5C8
Year 2012
Type of Item Peer-Reviewed Journal Publication
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Bibliographic Citation C.V. Chrysikopoulos , V. I. Syngouna ,I. A. Vasiliadou ,V. E. Katzourakis, "Transport of pseudomonas putida in a 3-D bench scale experimental aquifer " Transp. in Por Media , vol. 94 ,no.3, pp. 617-642 ,2012.doi:10.1007/s11242-012-0015-z https://doi.org/10.1007/s11242-012-0015-z
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Summary

This study is focused on the transport of Pseudomonas (P.) putida bacterial cellsin a 3-D model aquifer. The pilot-scale aquifer consisted of a rectangular glass tank withinternal dimensions: 120 cm length, 48 cm width, and 50 cm height, carefully packed withwell-characterized quartz sand. The P. putida decay was adequately represented by a firstordermodel. Transport experiments with a conservative tracer and P. putida were conductedto characterize the aquifer and to investigate the bacterial behavior during transport in watersaturated porous media. A 3-D, finite-difference numerical model for bacterial transport insaturated, homogeneous porous media was developed and was used to successfully fit theexperimental data. Furthermore, theoretical interaction energy calculations suggested thatthe extended-DLVO theory seems to predict bacteria attachment onto the aquifer sand betterthan the classical DLVO theory.

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