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Cotransport of Pseudomonas putida and kaolinite particles throughwater-saturated columns packed with glass bead

Chrysikopoulos Constantinos, Ioanna A. Vasiliadou

Πλήρης Εγγραφή

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Περίληψη

This study is focused on Pseudomonas putida bacteria transport in porous media in thepresence of suspended kaolinite clay particles. Experiments were performed with bacteriaand kaolinite particles separately to determine their individual transport characteristics inwater-saturated columns packed with glass beads. The results indicated that the massrecovery of bacteria and clay particles decreased as the pore water velocity decreased.Batch experiments were carried out to investigate the attachment of Pseudomonas putidaonto kaolinite particles. The attachment process was adequately described by a Langmuirisotherm. Finally, bacteria and kaolinite particles were injected simultaneously into apacked column in order to investigate their cotransport behavior. The experimental datasuggested that the presence of clay particles significantly inhibited the transport of bacteriain water-saturated porous media. The observed reduction of Pseudomonas putida recoveryin the column outflow was attributed to bacteria attachment onto kaolinite particles, whichwere retained onto the solid matrix of the column. A mathematical model was developed todescribe the transport of bacteria in the presence of suspended clay particles in onedimensionalwater-saturated porous media. Model simulations were in good agreement withthe experimental results.

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